Trump has lost almost $1.5 billion in net worth in two years.
Trump's net worth, which is largely reliant on his vast property portfolio, dropped by $600m (£455m) to just $3.1bn.
At the top of the list for the 24th consecutive year is the Microsoft founder and serial philanthropist Bill Gates, who has an estimated value of $89bn.
His highest net worth valuation by the magazine came in 2015 at $4.5 billion.
The list is out of Forbes 400 Richest Americans and it shows that the commander in chief's wealth dropped by $600 million to $3.1 billion.
Not every billionaire in the U.S. was rich enough to make the list.Brendan Hoffman / GettyOn Tuesday, Forbes released its 2017 list of the richest people in America.
"A tough NY real estate market, particularly for retail locations; a costly lawsuit and an expensive presidential campaign all contributed to the declining fortune of the 45th president", wrote Forbes. By Forbes' estimates, Trump's wealth has fallen 31% in two years.
Nonetheless, Trump gave Forbes a White House interview earlier this month in which the magazine reported he discussed his relationship with Secretary of State Rex Tillerson, a new economic development plan, and plans not to fill vacancies within the White House administration.
Forbes reports that Trump's most valuable assets remain New York City real estate holdings at $1.6 billion, followed by his golf course and resort properties valued at $570 million, and then non-New York real estate at $500 million.
Where did the Trump net worth see the biggest losses?
A total of 169 billionaires were not rich enough to make the list.