The attack took place on Route 60 next to Gush Etzion junction.
A spokesperson for Jerusalem's Shaare Zedek Medical Center said the Palestinian assailant was brought to the hospital in critical condition after receiving treatment on the scene by military medics.
Israeli troops shot and killed a Palestinian driver who rammed his vehicle into a bus stop in the occupied West Bank on Wednesday injuring a pedestrian, the army and a hospital spokesman said. The Palestinians say it is the result of 50 years of Israeli occupation of the West Bank and the Gaza Strip, home to more than five million Palestinians.
The attack came days after a Palestinian fatally stabbed British exchange student Hannah Bladon on Jerusalem's light rail.
A man of about 60, attacked by a Palestinian terrorist in Gush Etzion, south of Jerusalem, is being treated at a Jerusalem hospital.
In that same time, Israeli forces killed at least 244 Palestinians, a lot of them identified as attackers by Israeli authorities.
The incident was the latest in a spate of violence between Israelis and Palestinians that have claimed lives of at least 241 Palestinians, 41 Israelis, two US nationals, a British tourist, and two African asylum seekers since September 2015.